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Indoor Air Testing For Mould

There are a number of methods that can be used to test air for airborne mould spores and fragments. The method chosen would depend on the objective of the investigation and subsequently the type of data required. The following methods can be used for testing air for airborne mould contamination.

Settle Plate Method For Detecting Viable Airborne Mould Spores And Fragments
With this method, a set of plates containing suitable growth media is exposed at table‑top level for half-hour to 4 hours. The mould spores and fragments suspended in the air settle by gravity onto the growth media. Light particles that remain suspended in the air for many hours are likely to be missed out. However, it is the cheapest way of sampling air since no other equipment is needed.

Volumetric Air Sampling For Detecting Viable Airborne Mould Spores And Fragments
The settle plate method is generally not recommended. A better method involves impacting a known amount of air on some suitable growth media. The media could be liquid or solid. With this method viable spores or vegetative material would form visible colonies (referred to colony forming units) in the media. These are counted in the lab and expressed as colony forming units (CFU) per cubic meter of air.

Volumetric Air Sampling For Detecting Total Airborne Mould Spores And Fragments
One disadvantage of impacting air on growth media is that only the viable propagules can grow on the media and hence detected. It is estimated that only about 10-15% of the mould spores may be viable. To enable us have a better estimation of how contaminated the air is, air is drawn on onto some inert sticky stuff. Depending on the efficiency of the sampling equipment used most of the spores and other airborne particulate matter including insect parts, pollen grain, and all sorts of fibres are trapped onto the sticky surface. Since these samples are directly examined under a microscope, the analyst is able to count the spores and vegetative fragments and express them as spores per cubic meter of air. It is also possible to identify some of the spores to genus level.

For more information on mould sampling visit our web page http://www.moldbacteria.com/sendingsamples.html.

What Are Acceptable Levels Of Mould Spores And What Are The Effects Of Spores On The Human Body?

After analysing air samples for total spore counts, we often get asked whether there are acceptable levels of mould concentrations in air and what effects mould spores have on human health. Unlike many other indoor pollutants, there are currently no set acceptable levels for mould spores. The scientific community including medical doctors is polarized on the effects of mould on human health. However, it is generally accepted that mould can:

  • Elicit allergic reactions to sensitized (or atopic) individuals and that not all moulds are allergenic
  • Infect humans especially those with weak immune systems
  • Produce toxic metabolites (mycotoxins) that can kill or cause other complications if ingested in food.

While scientific evidence to prove that mould is the cause of some illnesses is lacking, there is enough evidence to support the need to remove mould from occupied environments.

Salmonella food poisoning and symptoms

Salmonella food poisoning has been a growing concern in recent news. It has frequently lead to recalls of beef, salads and organic products and, even peanut butter products. Salmonella food poisoning occurs when one eats raw or undercooked foods such meat, poultry, eggs or egg products. Properly cooked food can get contaminated with Salmonella if […]

Legionella pneumophila – Guidelines for Laboratory Interpretation

Legionella pneumophila is a Gram negative, aerobic bacteria that is characterized as an opportunistic pathogen. It is the cause of Legionnaires’ Disease, a severe form of pneumonia and, it is the cause of Pontiac fever, a non-pneumonic form of L. pneumophila infection. Legionella spp.’s mode of transmission is through aerosols or aspiration of contaminated water. The Public Health […]

The Indoor Mold Is An Early Warning Device

The mold (or mould, in the Queen’s English) has been the victim of a bad press. Mention the word and it conjures up an image of something rotting or in the process of decomposition. And yet, were it not for the mold that inspired the discovery of penicillin millions of lives would have been lost. […]