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You are here: Home / Archives for Fungi

Fungal investigations in public buildings, workplaces, and homes

Jackson Kung'u

Investigation of fungal contamination in indoor environments normally includes visual inspection and sampling. The samples to collect, the number, when and where to collect them and the methods to be used for sample analyses depends on the objectives or goal of the investigation. The samples that may be collected include air, dust or bulk samples. Swabs or clear cellophane tape can be used to sample for fungi from contaminated surfaces. The samples can be analyzed by either direct microscopy or by culture methods depending on the type of data required.

Filed Under: Fungi, Microbial Sampling Tagged With: air, bulk, dust, fungal contamination, Fungi, samples, swabs

Indoor Air Quality: Biological Pollutants

Jackson Kung'u

Indoor air quality is compromised by both biological and non-biological pollutants. Sources of biological pollutants include microorganisms, animal, insects and related organisms.

The most common biological pollutants of indoor air quality include molds and their byproducts, dust mites, pet dander (scales from hair, feathers, or skin), droppings and body parts from cockroaches, rodents and other pests or insects, viruses, and bacteria. Due to their small size, many of these biological pollutants are airborne and easily inhaled deep into the lungs.

Health Effects From Poor Indoor Air Quality

Some biological pollutants may cause serious health problems including hypersensitivity pneumonitis, allergic rhinitis, and some types of asthma. Other health problems attributed to biological pollutants are sneezing, watery eyes, coughing, shortness of breath, dizziness, lethargy, fever, and digestive problems.

It is believed allergic reactions occur only after repeated exposure to a specific biological allergen. The reaction may occur immediately upon re-exposure or after multiple exposures over time. It’s important to regularly monitor the indoor air quality.

Filed Under: Fungi, Indoor Air Quality Tagged With: Bacteria, biological pollutants, Fungi, health risks, Indoor Air Quality, insects, microorganisms

Colony Forming Units (CFU)

Jackson Kung'u

Colony Forming Units - CFUWhat are colony forming units? Colony forming units, usually abbreviated as CFU, refer to individual colonies of bacteria, yeast or mold. A colony of bacteria or yeast refers to a mass of individual cells of same organism, growing together. For moulds, a colony is a group of hyphae (filaments) of the same mould growing together. Colony forming units are used as a measure of the number of microorganisms present in or on surface of a sample. Colony forming units may be reported as CFU per unit weight, CFU per unit area, or CFU per unit volume depending on the type of sample tested. To determine the number of colony forming units, a sample is prepared and spread or poured uniformly on a surface of an agar plate and then incubated at some suitable temperature for a number of days. The colonies that form are counted. CFU is not a measure for individual cells or spores as a colony may be formed from a single or a mass of cells or spores.

Filed Under: Bacteria, Fungi Tagged With: Bacteria, cfu, colony forming units, Fungi, Yeast

My Interest In The Study Of Fungi.

Jackson Kung'u

I have always been fascinated by fungi. Fungi are a group of organisms to which moulds (mildews), yeasts, and mushrooms belong. Fungi used to be classified under the plant kingdom. However, they differ from plants in that they cannot make their own food and their cell wall is mainly made of chitin and glucan and not cellulose. Since they cannot make their own food, fungi have to survive as either saprophytes, parasites (pathogens) or by forming symbiotic relationships with their hosts. Saprophytic moulds are the ones commonly found in indoor environment growing on wall surfaces and other organic substrates. It is estimated that there are close to 1.5 million species of fungi but only about 100,000 species have been described.

Entomosporium spores
Entomosporium spores

My interest in fungi started when I completed my B.Sc. (Agriculture) and got employed as a plant pathologist. As a trainee plant pathologist, I spent countless hours in a plant clinic where farmers brought their diseased plants for diagnoses. My first fungus to identify without help was Entomosporium. One feature that makes me remember this fungus is the shape of the spores. The spores have appendages that make them resemble insects or two-legged mice with legs stretched sideways. I also went out into the field collecting diseased plants and taking them back to the laboratory for disease diagnoses and preservation as reference material. Working with diseased plants is not always easy because they won’t tell you where it is hurting (if at all they feel pain). You have to depend on symptoms and laboratory culturing and identification of the causative agent. If a plant is just wilting, for example, it is had to tell the cause of wilting which could be due to fungi, nematodes, bacteria, physical/chemical or physiological factors that interfere with water movement in the plant.

In my early days as a trainee, one challenge I had was to isolate the disease causing agent and getting pure cultures of the mould. It was not always easy because of the myriad saprophytic moulds and bacteria present on the surfaces of the plant. However, I learned quickly some clever ways plant pathologists and mycologists have developed to overcome the problem of contamination.

I was fortunate to be taught and interact with some of the world authorities in Mycology at CABI Bioscience (formerly the International Mycological Institute) and the University of Kent in the United Kingdom. These great people made me to like the subject.

My interest in fungi is now focused on indoor moulds. Indoor moulds are recognized health hazards and it is estimated that 20% of human population is sensitive to mould exposure. It is also believed that long-term exposure to mould could lead to sensitization.

Filed Under: Fungi Tagged With: Fungi, mildews, moulds, yeasts

Which Are The Common Indoor Fungi?

Jackson Kung'u

Close to 100,000 species of fungi have been described. However, only a small number has been reported indoors. The most common indoor fungi include some species of Cladosporium, Aspergillus, and Penicillium. These may be found growing on damp wall surfaces in the basement, washroom, kitchen, windowsills, and ceiling tiles.

Below is a list of fungi that have been found in indoor environment. Click the name of the fungus (if the link is active) to get some details about its ecology and associated health effects where known.

Fungi that have been reported from indoor environment

Absidia corymbifera

Acremonium Species
Acremonium strictum

Alternaria Species
Alternaria alternata

Aspergillus Species
Aspergillus candidus
Aspergillus clavatus
Aspergillus flavipes
Aspergillus flavus
Aspergillus fumigatus.
Aspergillus nidulans
Aspergillus niger.
Aspergillus niveus.
Aspergillus ochraceous.
Aspergillus penicillioides
Aspergillus restrictus
Aspergillus sydowii
Aspergillus tamarii
Aspergillus terreus.
Aspergillus ustus
Aspergillus versicolor
Aspergillus wentii

Aureobasidium pullulans

Chaetomium globosum

Chrysonilia Species
Chrysonilia crassa
Chrysonilia sitophila

Cladosporium Species
Cladosporium cladosporioides
Cladosporium herbarum
Cladosporium sphaerospermum

Curvularia Species
Curvularia aureum
Curvularia geniculata
Curvularia lunata

Emericella nidulans (=Aspergillus nidulans)

Epicoccum nigrum

Eurotium Species
Eurotium amstelodami
Eurotium chevalieri
Eurotium herbariorum

Exophiala jeanselmei group

Fusarium Species
Fusarium culmorum
Fusarium oxysporum
Fusarium solani
Fusarium verticillioides (= Fusarium moniliforme)

Geomyces pannorum.

Geotrichum candidum

Gliocladium Species

Gliomastix murorum

Memnoniella echinulata

Mucor Species
Mucor hiemalis
Mucor mucedo
Mucor plumbeus
Mucor racemosus

Myrothecium verrucaria

Oidiodendron Species
Oidiodendron griseum
Oidiodendron tenuissimum

Paecilomyces Species
Paecilomyces lilacinus
Paecilomyces variotii

Penicillium Species
Penicillium aurantiogriseum
Penicillium brevicompactum
Penicillium chrysogenum
Penicillium citrinum.
Penicillium commune
Penicillium corylophilum
Penicillium crustosum
Penicillium expansum
Penicillium funiculosum
Penicillium glabrum
Penicillium janthinellum
Penicillium olsonii
Penicillium polonicum
Penicillium roqueforti
Penicillium rugulosum
Penicillium simplicissimum
Penicillium spinulosum
Penicillium variabile.
Penicillium viridicatum

Phialophora Species
Phialophora fastigiata

Phoma Species
Phoma glomerata
Phoma macrostoma

Pithomyces Species
Pithomyces chartarum
Pithomyces Species

Rhizopus stolonifer

Scopulariopsis Species
Scopulariopsis brevicaulis
Scopulariopsis candida
Scopulariopsis fusca
Scopulariopsis Species

Sistotrema brinkmanii.

Sporothrix Species
Sporothrix schenckii.

Stachybotrys chartarum

Stemphylium botryosum

Syncephalastrum racemosum

Trichoderma Species
Trichoderma harzianum
Trichoderma viride

Ulocladium Species
Ulocladium botrytis
Ulocladium chartarum

Wallemia sebi

 

Mold References

Filed Under: Fungi Tagged With: Aspergillus, cladosporium, Fungi, penicillium

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